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PIK3R2 Recombinant Rabbit mAb

#3343

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Recombinant Antibody
Western blotting analysis using PI3 Kinase p85 beta antibody (Cat#3343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p85 beta antibody (Cat#3343, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PI3 Kinase p85 beta expression in HepG2 cells using PI3 Kinase p85 beta antibody (Cat#3343, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 Kinase p85 beta.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PIK3R2
  • Catalog:
    3343
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PIK3R2 Recombinant Rabbit mAb
Aliases PIK3R2; Phosphoinositide-3-Kinase Regulatory Subunit 2; P85-BETA; P85beta; P85B; P85; Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Beta; Phosphoinositide-3-Kinase, Regulatory Subunit 2 (Beta); Phosphatidylinositol 3-Kinase Regulatory Subunit Beta; Phosphoinositide-3-Kinase Regulatory Subunit Beta; PtdIns-3-Kinase Regulatory Subunit P85-Beta; PI3K Regulatory Subunit Beta; PI3-Kinase Subunit P85-Beta; Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 2 (P85 Beta); PtdIns-3-Kinase Regulatory Subunit Beta; PI3-Kinase Regulatory Subunit Beta; MPPH1; MPPH
Background

Gene Name: PIK3R2

NCBI Gene Entry: 5296

UniProt Entry: O00459

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 82 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5210

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PI 3 Kinase p85 beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PI3 Kinase p85 beta antibody (Cat#3343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p85 beta antibody (Cat#3343, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PI3 Kinase p85 beta expression in HepG2 cells using PI3 Kinase p85 beta antibody (Cat#3343, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 Kinase p85 beta.
Western blotting analysis using PI3 Kinase p85 beta antibody (Cat#3343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p85 beta antibody (Cat#3343, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PI3 Kinase p85 beta expression in HepG2 cells using PI3 Kinase p85 beta antibody (Cat#3343, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 Kinase p85 beta.
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