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Home>Product>Primary Antibody

P2Y12 Recombinant Rabbit mAb

#2035

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Recombinant Antibody
Western blotting analysis using P2Y12 antibody (Cat#2035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P2Y12 antibody (Cat#2035, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with P2Y12 antibody (Cat#2035, 1:1,000). Nuclei were stained blue with DAPI; P2Y12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of P2Y12 expression in HepG2 cells using P2Y12 antibody (Cat#2035, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, P2Y12.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    P2RY12
  • Catalog：
    2035
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name P2Y12 Recombinant Rabbit mAb
Aliases P2RY12; Purinergic Receptor P2Y12; SP1999; P2Y12; HORK3; Purinergic Receptor P2Y, G-Protein Coupled, 12; P2Y12 Platelet ADP Receptor; ADP-Glucose Receptor; P2Y Purinoceptor 12; P2Y(ADP); P2Y(Cyc); P2T(AC); P2Y(AC); ADPG-R; Putative G-Protein Coupled Receptor; G-Protein Coupled Receptor SP1999; Gi-Coupled ADP Receptor HORK3; Purinergic Receptor P2RY12; P2Y(12)R; BDPLT8
Background

Gene Name: P2RY12

NCBI Gene Entry: 64805

UniProt Entry: Q9H244

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4970

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human P2Y12
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using P2Y12 antibody (Cat#2035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P2Y12 antibody (Cat#2035, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with P2Y12 antibody (Cat#2035, 1:1,000). Nuclei were stained blue with DAPI; P2Y12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of P2Y12 expression in HepG2 cells using P2Y12 antibody (Cat#2035, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, P2Y12.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    P2RY12
  • Catalog：
    2035
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using P2Y12 antibody (Cat#2035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with P2Y12 antibody (Cat#2035, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with P2Y12 antibody (Cat#2035, 1:1,000). Nuclei were stained blue with DAPI; P2Y12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of P2Y12 expression in HepG2 cells using P2Y12 antibody (Cat#2035, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, P2Y12.
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