Western blotting analysis using ARG1 antibody (Cat#6549). Total lysates (30 μg) from mouse liver tissue were loaded and separated by SDS-PAGE. The blot was incubated with ARG1 antibody (Cat#6549, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ARG1 antibody (Cat#6549, 1:1,000). Nuclei were stained blue with DAPI; ARG1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High . Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARG1 expression in HepG2 cells using ARG1 antibody (Cat#6549, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARG1.
