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Home>Product>Primary Antibody

KD-Validated BUB1B Rabbit pAb

#62829

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shRNA Knockdown Validated
Western blotting analysis using BUB1B antibody (Cat#62829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB1B antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BUB1B kinase antibody (Cat#62829). BUB1B kinase expression in wild type (WT) and BUB1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BUB1B kinase antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BUB1B
  • Catalog：
    62829
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated BUB1B Rabbit pAb
Aliases BUB1B; BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; BUBR1; MAD3L; SSK1; Bub1A; Mitotic Checkpoint Serine/Threonine-Protein Kinase BUB1 Beta; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast); Budding Uninhibited By Benzimidazoles 1 Homolog Beta; BUB1B, Mitotic Checkpoint Serine/Threonine Kinase; Protein SSK1; EC 2.7.11.1; BUB1beta; MVA1
Background

Gene Name: BUB1B

NCBI Gene Entry: 701

UniProt Entry: O60566

Application Information

Molecular Weight: Predicted, 120 kDa; observed, 130 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human BUB1B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using BUB1B antibody (Cat#62829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB1B antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using BUB1B kinase antibody (Cat#62829). BUB1B kinase expression in wild type (WT) and BUB1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BUB1B kinase antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BUB1B
  • Catalog：
    62829
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using BUB1B antibody (Cat#62829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB1B antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BUB1B kinase antibody (Cat#62829). BUB1B kinase expression in wild type (WT) and BUB1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BUB1B kinase antibody (Cat#62829, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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