Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

Phospho-Smad1 (Ser463/Ser465) Recombinant Rabbit mAb

#3330

Click to copy product information

Return to List

Recombinant Antibody
Western blotting analysis using phospho-smad1 (Ser463/Ser465) antibody (Cat#3330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-smad1 (Ser463/Ser465) antibody (Cat#3330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Smad1 (Ser463/Ser465) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Smad1 (Ser463/Ser465) expression in HAP-1 cells using Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Smad1 (Ser463/Ser465).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMAD1
  • Catalog：
    3330
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Smad1 (Ser463/Ser465) Recombinant Rabbit mAb
Aliases SMAD1; SMAD Family Member 1; MADR1; JV4-1; MADH1; Mothers Against Decapentaplegic Homolog 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; BSP-1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1; SMAD, Mothers Against DPP Homolog 1 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 1; SMAD, Mothers Against DPP Homolog 1; TGF-Beta Signaling Protein 1; MAD Homolog 1; SMAD 1; HSMAD1; Smad1; JV41
Background

Gene Name: SMAD1

NCBI Gene Entry: 4086

UniProt Entry: Q15797

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5090

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Smad1 (Ser463/Ser465)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-smad1 (Ser463/Ser465) antibody (Cat#3330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-smad1 (Ser463/Ser465) antibody (Cat#3330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Smad1 (Ser463/Ser465) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-Smad1 (Ser463/Ser465) expression in HAP-1 cells using Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Smad1 (Ser463/Ser465).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMAD1
  • Catalog：
    3330
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-smad1 (Ser463/Ser465) antibody (Cat#3330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-smad1 (Ser463/Ser465) antibody (Cat#3330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Smad1 (Ser463/Ser465) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Smad1 (Ser463/Ser465) expression in HAP-1 cells using Phospho-Smad1 (Ser463/Ser465) antibody (Cat#3330, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Smad1 (Ser463/Ser465).
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.