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HSP90AA1 Mouse mAb

#3422

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Western blotting analysis using HSP90AA1 antibody (Cat#3422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AA1 antibody (Cat#3422, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of HSP90AA1 expression in HepG2 cells using HSP90AA1 antibody (Cat#3422, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSP90AA1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    HSP90AA1
  • Catalog:
    3422
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name HSP90AA1 Mouse mAb
Aliases HSP90AA1; Heat Shock Protein 90 Alpha Family Class A Member 1; HSP90N; HSPC1; HSPCA; Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1; Lipopolysaccharide-Associated Protein; Heat Shock 90kDa Protein 1, Alpha; Renal Carcinoma Antigen NY-REN-38; Heat Shock 90kD Protein 1, Alpha; Heat Shock Protein HSP 90-Alpha; LPS-Associated Protein; Heat Shock 86 KDa; FLJ31884; HSP90A; HSP 86; Hsp89; Hsp90; HSP86; LAP-2; Heat Shock Protein 90kDa Alpha Family Class A Member 1; Epididymis Secretory Sperm Binding Protein Li 65p; Epididymis Luminal Secretory Protein 52; Heat Shock 90kD Protein 1, Alpha-Like 4; Heat Shock 90kD Protein, Alpha-Like 4; EC 3.6.4.10; HEL-S-65p; HSPCAL1; HSPCAL4; HSP89A; Hsp103; HSP89; HSP90; EL52; HSPN; LAP2
Background

Gene Name: HSP90AA1

NCBI Gene Entry: 3320

UniProt Entry: P07900

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 85 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5550

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human HSP90AA1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using HSP90AA1 antibody (Cat#3422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AA1 antibody (Cat#3422, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
  • Flow cytometric analysis of HSP90AA1 expression in HepG2 cells using HSP90AA1 antibody (Cat#3422, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSP90AA1.
Western blotting analysis using HSP90AA1 antibody (Cat#3422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AA1 antibody (Cat#3422, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of HSP90AA1 expression in HepG2 cells using HSP90AA1 antibody (Cat#3422, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSP90AA1.
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