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Home>Product>Primary Antibody

Phospho-FAK (Y397) Recombinant Rabbit mAb

#3344

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Recombinant Antibody
Western blotting analysis using phospho-FAK (Y397) antibody (Cat#3344). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-FAK (Y397) antibody (Cat#3344, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with phospho-FAK (Y397) antibody (Cat#3344, 1:1,000). Nuclei were stained blue with DAPI; Phospho-FAK (Y397) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-FAK (Y397) expression in HepG2 cells using phospho-FAK (Y397) antibody (Cat#3344, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-FAK (Y397).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTK2
  • Catalog：
    3344
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-FAK (Y397) Recombinant Rabbit mAb
Aliases PTK2; Protein Tyrosine Kinase 2; PPP1R71; FAK1; FAK; FADK; Protein Phosphatase 1 Regulatory Subunit 71; Focal Adhesion Kinase-Related Nonkinase; PTK2 Protein Tyrosine Kinase 2; Focal Adhesion Kinase 1; EC 2.7.10.2; Pp125FAK; P125FAK; FADK; FRNK; Protein Phosphatase 1, Regulatory Subunit 71; FAK-Related Non-Kinase Polypeptide; Protein-Tyrosine Kinase; EC 2.7.10
Background

Gene Name: PTK2

NCBI Gene Entry: 5747

UniProt Entry: Q05397

Application Information

Molecular Weight: Predicted, 119 kDa; observed, 119 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5215

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-FAK (Y397)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-FAK (Y397) antibody (Cat#3344). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-FAK (Y397) antibody (Cat#3344, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with phospho-FAK (Y397) antibody (Cat#3344, 1:1,000). Nuclei were stained blue with DAPI; Phospho-FAK (Y397) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of phospho-FAK (Y397) expression in HepG2 cells using phospho-FAK (Y397) antibody (Cat#3344, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-FAK (Y397).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTK2
  • Catalog：
    3344
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-FAK (Y397) antibody (Cat#3344). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-FAK (Y397) antibody (Cat#3344, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with phospho-FAK (Y397) antibody (Cat#3344, 1:1,000). Nuclei were stained blue with DAPI; Phospho-FAK (Y397) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-FAK (Y397) expression in HepG2 cells using phospho-FAK (Y397) antibody (Cat#3344, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-FAK (Y397).
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