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Home>Product>Primary Antibody

ATP2B1 Recombinant Rabbit mAb

#3355

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Recombinant Antibody
Western blotting analysis using ATP2B1 antibody (Cat#3355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2B1 antibody (Cat#3355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ATP2B1 antibody (Cat#3355, 1:1,000). Nuclei were stained blue with DAPI; ATP2B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP2B1 expression in HepG2 cells using ATP2B1 antibody (Cat#3355, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP2B1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP2B1
  • Catalog：
    3355
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ATP2B1 Recombinant Rabbit mAb
Aliases ATP2B1; ATPase Plasma Membrane Ca2+ Transporting 1; PMCA1; Plasma Membrane Calcium-Transporting ATPase 1; ATPase, Ca++ Transporting, Plasma Membrane 1; Plasma Membrane Calcium ATPase Isoform 1; Plasma Membrane Calcium Pump Isoform 1; Plasma Membrane Calcium Pump; EC 7.2.2.10; EC 3.6.3.8; EC 3.6.3; PMCA1kb; MRD66
Background

Gene Name: ATP2B1

NCBI Gene Entry: 490

UniProt Entry: P20020

Application Information

Molecular Weight: Predicted, 135 kDa; observed, 130-250 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5270

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PMCA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP2B1 antibody (Cat#3355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2B1 antibody (Cat#3355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with ATP2B1 antibody (Cat#3355, 1:1,000). Nuclei were stained blue with DAPI; ATP2B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ATP2B1 expression in HepG2 cells using ATP2B1 antibody (Cat#3355, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP2B1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP2B1
  • Catalog：
    3355
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP2B1 antibody (Cat#3355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2B1 antibody (Cat#3355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ATP2B1 antibody (Cat#3355, 1:1,000). Nuclei were stained blue with DAPI; ATP2B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP2B1 expression in HepG2 cells using ATP2B1 antibody (Cat#3355, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP2B1.
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