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DBF4 Recombinant Rabbit mAb

#3374

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Recombinant Antibody
Western blotting analysis using DBF4 antibody (Cat#3374). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DBF4 antibody (Cat#3374, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DBF4 expression in HepG2 cells using DBF4 antibody (Cat#3374, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,DBF4.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    DBF4
  • Catalog:
    3374
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name DBF4 Recombinant Rabbit mAb
Aliases DBF4; DBF4-CDC7 Kinase Regulatory Subunit; ZDBF1; DBF4A; ASK; Activator Of S Phase Kinase; Chif; DBF4-Type Zinc Finger-Containing Protein 1; Zinc Finger, DBF-Type Containing 1; Regulatory Subunit Of DDK; Protein DBF4 Homolog A; DBF4 Zinc Finger A; Chiffon Homolog A; DBF4 Zinc Finger; Chiffon Homolog (Drosophila); DBF4 Homolog (S. Cerevisiae); DBF4 Homolog
Background

Gene Name: DBF4

NCBI Gene Entry: 10926

UniProt Entry: Q9UBU7

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 77 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5370

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human DBF4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using DBF4 antibody (Cat#3374). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DBF4 antibody (Cat#3374, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of DBF4 expression in HepG2 cells using DBF4 antibody (Cat#3374, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,DBF4.
Western blotting analysis using DBF4 antibody (Cat#3374). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DBF4 antibody (Cat#3374, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DBF4 expression in HepG2 cells using DBF4 antibody (Cat#3374, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,DBF4.
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