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GRIN2A Recombinant Rabbit mAb

#3686

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Recombinant Antibody
Western blotting analysis using grin2A antibody (Cat#3686). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with grin2A antibody (Cat#3686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Grin2A antibody (Cat#3686, 1:1,000). Nuclei were stained blue with DAPI; Grin2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Grin2A expression in HepG2 cells using Grin2A antibody (Cat#3686, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Grin2A.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    GRIN2A
  • Catalog:
    3686
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name GRIN2A Recombinant Rabbit mAb
Aliases GRIN2A; Glutamate Ionotropic Receptor NMDA Type Subunit 2A; GluN2A; NMDAR2A; NR2A; Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2A; N-Methyl D-Aspartate Receptor Subtype 2A; Glutamate Receptor Ionotropic, NMDA 2A; N-Methyl-D-Aspartate Receptor Channel, Subunit Epsilon-1; Glutamate [NMDA] Receptor Subunit Epsilon-1 N-Methyl-D-Aspartate Receptor Subunit 2A; Glutamate Ionotropic Receptor NMDA 2A; HNR2A; EPND; FESD; LKS
Background

Gene Name: GRIN2A

NCBI Gene Entry: 2903

UniProt Entry: Q12879

Application Information

Molecular Weight: Predicted, 165 kDa; observed, 250 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6905

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of mouse NMDAR2A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using grin2A antibody (Cat#3686). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with grin2A antibody (Cat#3686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Grin2A antibody (Cat#3686, 1:1,000). Nuclei were stained blue with DAPI; Grin2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Grin2A expression in HepG2 cells using Grin2A antibody (Cat#3686, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Grin2A.
Western blotting analysis using grin2A antibody (Cat#3686). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with grin2A antibody (Cat#3686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Grin2A antibody (Cat#3686, 1:1,000). Nuclei were stained blue with DAPI; Grin2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Grin2A expression in HepG2 cells using Grin2A antibody (Cat#3686, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Grin2A.
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