Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

Caspase 8 Mouse mAb

#3750

Click to copy product information

Return to List

Western blotting analysis using caspase 8 antibody (Cat#3750). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody (Cat#3750, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using caspase 8 antibody (Cat#3750, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Caspase 8 expression in HeLa cells using Caspase 8 antibody (Cat#3750, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP8
  • Catalog：
    3750
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name Caspase 8 Mouse mAb
Aliases CASP8; Caspase 8; FLICE; MCH5; MACH; Casp-8; Caspase 8, Apoptosis-Related Cysteine Peptidase; Caspase 8, Apoptosis-Related Cysteine Protease; MORT1-Associated Ced-3 Homolog; ICE-Like Apoptotic Protease 5; Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; FADD-Like ICE; Caspase-8; CAP4; FADD-Homologous ICE/CED-3-Like Protease; FADD-Homologous ICE/Ced-3-Like Protease; MACH-Beta-1/2/3/4 Protein; MACH-Alpha-1/2/3 Protein; EC 3.4.22.61; ALPS2B; CASP-8
Background

Gene Name: CASP8

NCBI Gene Entry: 841

UniProt Entry: Q14790

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7195

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Caspase 8
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100
Note This product is for research use only.
Data
  • Western blotting analysis using caspase 8 antibody (Cat#3750). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody (Cat#3750, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using caspase 8 antibody (Cat#3750, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Caspase 8 expression in HeLa cells using Caspase 8 antibody (Cat#3750, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP8
  • Catalog：
    3750
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using caspase 8 antibody (Cat#3750). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody (Cat#3750, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using caspase 8 antibody (Cat#3750, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Caspase 8 expression in HeLa cells using Caspase 8 antibody (Cat#3750, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 8.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.