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Double PHD Fingers 2 Mouse mAb

#3754

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Western blotting analysis using double PHD fingers 2 antibody (Cat#3754). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with double PHD fingers 2 antibody (Cat#3754, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Double PHD fingers 2 expression in HT-1080 cells using Double PHD fingers 2 antibody (Cat#3754, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Double PHD fingers 2.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    DPF2
  • Catalog:
    3754
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name Double PHD Fingers 2 Mouse mAb
Aliases DPF2; Double PHD Fingers 2; D4, Zinc And Double PHD Fingers Family 2; SMARCG2; Ubi-D4; BAF45d; REQ; Apoptosis Response Zinc Finger Protein; Zinc Finger Protein Ubi-D4; BRG1-Associated Factor 45D; Protein Requiem; UBID4; Requiem, Apoptosis Response Zinc Finger Gene; Requiem, Apoptosis Response Zinc Finger; BAF45D; CSS7
Background

Gene Name: DPF2

NCBI Gene Entry: 5977

UniProt Entry: Q92785

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 44 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7215

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human DPF2
Isotype Mouse IgG3 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using double PHD fingers 2 antibody (Cat#3754). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with double PHD fingers 2 antibody (Cat#3754, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Double PHD fingers 2 expression in HT-1080 cells using Double PHD fingers 2 antibody (Cat#3754, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Double PHD fingers 2.
Western blotting analysis using double PHD fingers 2 antibody (Cat#3754). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with double PHD fingers 2 antibody (Cat#3754, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Double PHD fingers 2 expression in HT-1080 cells using Double PHD fingers 2 antibody (Cat#3754, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Double PHD fingers 2.
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