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Dihydropyrimidinase Like 5 Mouse mAb

#3755

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Western blotting analysis using dihydropyrimidinase like 5 antibody (Cat#3755). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dihydropyrimidinase like 5 antibody (Cat#3755, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Dihydropyrimidinase like 5 antibody (Cat#3755, 1:1,000). Nuclei were stained blue with DAPI;Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using Dihydropyrimidinase like 5 antibody (Cat#3755, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Dihydropyrimidinase like 5.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    DPYSL5
  • Catalog:
    3755
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name Dihydropyrimidinase Like 5 Mouse mAb
Aliases DPYSL5; Dihydropyrimidinase Like 5; CRMP-5; CRMP5; CRAM; Ulip6; CV2; Collapsin Response Mediator Protein 5; Dihydropyrimidinase-Related Protein 5; UNC33-Like Phosphoprotein 6; CRMP3-Associated Molecule; ULIP-6; DRP-5; Collapsin Response Mediator Protein-5; Dihydropyrimidinase-Like 5; RTSC4; ULIP6
Background

Gene Name: DPYSL5

NCBI Gene Entry: 56896

UniProt Entry: Q9BPU6

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 61 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7220

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human CRMP-5
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using dihydropyrimidinase like 5 antibody (Cat#3755). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dihydropyrimidinase like 5 antibody (Cat#3755, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with Dihydropyrimidinase like 5 antibody (Cat#3755, 1:1,000). Nuclei were stained blue with DAPI;Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using Dihydropyrimidinase like 5 antibody (Cat#3755, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Dihydropyrimidinase like 5.
Western blotting analysis using dihydropyrimidinase like 5 antibody (Cat#3755). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dihydropyrimidinase like 5 antibody (Cat#3755, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Dihydropyrimidinase like 5 antibody (Cat#3755, 1:1,000). Nuclei were stained blue with DAPI;Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using Dihydropyrimidinase like 5 antibody (Cat#3755, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Dihydropyrimidinase like 5.
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