Western blotting analysis using beta Actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:10,000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using beta actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of β-Actin expression in H9c2 cells using β-Actin antibody (Cat#3793-HRP, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, β-Actin.
Western blotting analysis using beta Actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:10,000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using beta actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of β-Actin expression in H9c2 cells using β-Actin antibody (Cat#3793-HRP, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, β-Actin.
Western blotting analysis using beta Actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:10,000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using beta actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of β-Actin expression in H9c2 cells using β-Actin antibody (Cat#3793-HRP, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, β-Actin.
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