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Home>Product>Primary Antibody

Vascular Endothelial Growth Factor D Recombinant Rabbit mAb

#3920

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Recombinant Antibody
Western blotting analysis using vascular endothelial growth factor D antibody (Cat#3920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vascular endothelial growth factor D antibody (Cat#3920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse liver using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse heart using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of vascular endothelial growth factor D expression in HepG2 cells using vascular endothelial growth factor D antibody (Cat#3920, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, vascular endothelial growth factor D.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    VEGFD
  • Catalog：
    3920
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Vascular Endothelial Growth Factor D Recombinant Rabbit mAb
Aliases VEGFD; Vascular Endothelial Growth Factor D; VEGF-D; FIGF; C-Fos Induced Growth Factor (Vascular Endothelial Growth Factor D); C-Fos-Induced Growth Factor
Background

Gene Name: VEGFD

NCBI Gene Entry: 2277

UniProt Entry: O43915

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB8690

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human beta VEGFD
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using vascular endothelial growth factor D antibody (Cat#3920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vascular endothelial growth factor D antibody (Cat#3920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded mouse liver using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse heart using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of vascular endothelial growth factor D expression in HepG2 cells using vascular endothelial growth factor D antibody (Cat#3920, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, vascular endothelial growth factor D.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    VEGFD
  • Catalog：
    3920
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using vascular endothelial growth factor D antibody (Cat#3920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vascular endothelial growth factor D antibody (Cat#3920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse liver using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse heart using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using vascular endothelial growth factor D antibody (Cat#3920, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of vascular endothelial growth factor D expression in HepG2 cells using vascular endothelial growth factor D antibody (Cat#3920, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, vascular endothelial growth factor D.
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