• Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products

Trophoblast Glycoprotein Recombinant Rabbit mAb

#4099

Click to copy product information
Recombinant Antibody
Western blotting analysis using trophoblast glycoprotein antibody (Cat#4099). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with trophoblast glycoprotein antibody (Cat#4099, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using trophoblast glycoprotein antibody (Cat#4099, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of trophoblast glycoprotein expression in HAP-1 cells using trophoblast glycoprotein antibody (Cat#4099, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, trophoblast glycoprotein.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TPBG
  • Catalog:
    4099
  • Application:
    WB, FCM, IHC-P
  • Species Reactivity:
    H, M, R
Information
Product Name Trophoblast Glycoprotein Recombinant Rabbit mAb
Aliases TPBG; Trophoblast Glycoprotein; 5T4; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; 5T4-AG; WAIF1; M6P1; 5T4AG
Background

Gene Name: TPBG

NCBI Gene Entry: 7162

UniProt Entry: Q13641

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 72 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9585

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human 5T4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using trophoblast glycoprotein antibody (Cat#4099). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with trophoblast glycoprotein antibody (Cat#4099, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using trophoblast glycoprotein antibody (Cat#4099, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of trophoblast glycoprotein expression in HAP-1 cells using trophoblast glycoprotein antibody (Cat#4099, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, trophoblast glycoprotein.
Western blotting analysis using trophoblast glycoprotein antibody (Cat#4099). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with trophoblast glycoprotein antibody (Cat#4099, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using trophoblast glycoprotein antibody (Cat#4099, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of trophoblast glycoprotein expression in HAP-1 cells using trophoblast glycoprotein antibody (Cat#4099, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, trophoblast glycoprotein.
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.