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Carbonic Anhydrase 9 Recombinant Rabbit mAb

#4159

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Recombinant Antibody
Western blotting analysis using carbonic anhydrase 9 antibody (Cat#4159). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with carbonic anhydrase 9 antibody (Cat#4159, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Carbonic anhydrase 9 antibody (Cat#4159, 1:1,000). Nuclei were stained blue with DAPI; Carbonic anhydrase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CA9
  • Catalog:
    4159
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name Carbonic Anhydrase 9 Recombinant Rabbit mAb
Aliases CA9; Carbonic Anhydrase 9; CAIX; MN; Carbonic Anhydrase IX; Renal Cell Carcinoma-Associated Antigen G250; RCC-Associated Protein G250; RCC-Associated Antigen G250; Carbonate Dehydratase IX; Carbonic Dehydratase; Membrane Antigen MN; P54/58N; CA-IX; PMW1; EC 4.2.1.1; G250
Background

Gene Name: CA9

NCBI Gene Entry: 768

UniProt Entry: Q16790

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 33-58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9850

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human CA9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using carbonic anhydrase 9 antibody (Cat#4159). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with carbonic anhydrase 9 antibody (Cat#4159, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Carbonic anhydrase 9 antibody (Cat#4159, 1:1,000). Nuclei were stained blue with DAPI; Carbonic anhydrase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using carbonic anhydrase 9 antibody (Cat#4159). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with carbonic anhydrase 9 antibody (Cat#4159, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Carbonic anhydrase 9 antibody (Cat#4159, 1:1,000). Nuclei were stained blue with DAPI; Carbonic anhydrase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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