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Home>Product>Primary Antibody

cleaved Caspase-9 Recombinant Rabbit mAb

#4188

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Recombinant Antibody
Western blotting analysis using cleaved caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with cleaved caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cleaved Caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cleaved Caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#4188, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, cleaved Caspase-9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP9
  • Catalog：
    4188
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name cleaved Caspase-9 Recombinant Rabbit mAb
Aliases CASP9; Caspase 9; ICE-LAP6; APAF-3; MCH6; PPP1R56; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; Caspase-9; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; EC 3.4.22.62; CASP-9; APAF3
Background

Gene Name: CASP9

NCBI Gene Entry: 842

UniProt Entry: P55211

Application Information

Molecular Weight: Predicted, 35,46 kDa; observed, 35,37,46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9975

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human CASP9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using cleaved caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with cleaved caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using cleaved Caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cleaved Caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#4188, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, cleaved Caspase-9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP9
  • Catalog：
    4188
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using cleaved caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with cleaved caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cleaved Caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cleaved Caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#4188, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, cleaved Caspase-9.
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