Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#4268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-STAT3 (Tyr705) expression in H9c2 cells using phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-STAT3 (Tyr705).
STAT3; Signal Transducer And Activator Of Transcription 3; APRF; Acute-Phase Response Factor; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#4268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-STAT3 (Tyr705) expression in H9c2 cells using phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-STAT3 (Tyr705).
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#4268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-STAT3 (Tyr705) expression in H9c2 cells using phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-STAT3 (Tyr705).
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