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Home>Product>Primary Antibody

CD99 Recombinant Rabbit mAb

#4289

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Recombinant Antibody
Western blotting analysis using CD99 antibody (Cat#4289). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD99 antibody (Cat#4289, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using CD99 antibody (Cat#4289, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of CD99 expression in HepG2 cells using CD99 antibody (Cat#4289, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD99.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD99
  • Catalog：
    4289
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name CD99 Recombinant Rabbit mAb
Aliases CD99; CD99 Molecule (Xg Blood Group); CD99 Antigen; MIC2; Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13; T-Cell Surface Glycoprotein E2; E2 Antigen; MIC2X; MIC2Y; Antigen Identified By Monoclonal 12E7, Y Homolog; MIC2 (Monoclonal Antibody 12E7); Cell Surface Antigen HBA-71; Cell Surface Antigen 12E7; Cell Surface Antigen O13; Surface Antigen MIC2; CD99 Molecule; Protein MIC2; HBA71; MSK5X; 12E7
Background

Gene Name: CD99

NCBI Gene Entry: 4267

UniProt Entry: P14209

Application Information

Molecular Weight: Predicted, 19 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10475

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human CD99
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using CD99 antibody (Cat#4289). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD99 antibody (Cat#4289, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using CD99 antibody (Cat#4289, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of CD99 expression in HepG2 cells using CD99 antibody (Cat#4289, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD99.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD99
  • Catalog：
    4289
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using CD99 antibody (Cat#4289). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD99 antibody (Cat#4289, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using CD99 antibody (Cat#4289, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of CD99 expression in HepG2 cells using CD99 antibody (Cat#4289, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD99.
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