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IDH1 Recombinant Rabbit mAb

#4477

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Recombinant Antibody
Western blotting analysis using IDH1 antibody (Cat#4477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IDH1 antibody (Cat#4477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IDH1 expression in C2C12 cells using IDH1 antibody (Cat#4477, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IDH1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    IDH1
  • Catalog:
    4477
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name IDH1 Recombinant Rabbit mAb
Aliases IDH1; Isocitrate Dehydrogenase (NADP(+)) 1; Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic; Isocitrate Dehydrogenase 1 (NADP+), Soluble; Isocitrate Dehydrogenase [NADP] Cytoplasmic; Oxalosuccinate Decarboxylase; NADP(+)-Specific ICDH; EC 1.1.1.42; PICD; IDH; NADP-Dependent Isocitrate Dehydrogenase, Peroxisomal; NADP-Dependent Isocitrate Dehydrogenase, Cytosolic; Epididymis Secretory Sperm Binding Protein; Cytosolic NADP-Isocitrate Dehydrogenase; Isocitrate Dehydrogenase 1 (NADP+); Epididymis Secretory Protein Li 26; Epididymis Luminal Protein 216; HEL-S-26; HEL-216; IDCD; IDPC; IDPc; IDP
Background

Gene Name: IDH1

NCBI Gene Entry: 3417

UniProt Entry: O75874

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11415

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human IDH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using IDH1 antibody (Cat#4477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IDH1 antibody (Cat#4477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of IDH1 expression in C2C12 cells using IDH1 antibody (Cat#4477, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IDH1.
Western blotting analysis using IDH1 antibody (Cat#4477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IDH1 antibody (Cat#4477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IDH1 expression in C2C12 cells using IDH1 antibody (Cat#4477, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IDH1.
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