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Home>Product>Primary Antibody

Tribbles Pseudokinase 3 Recombinant Rabbit mAb

#4537

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Recombinant Antibody
Western blotting analysis using tribbles pseudokinase 3 antibody (Cat#4537). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tribbles pseudokinase 3 antibody (Cat#4537, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with Tribbles pseudokinase 3 antibody (Cat#4537, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of tribbles pseudokinase 3 expression in HT-1080 cells using tribbles pseudokinase 3 antibody (Cat#4537, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, tribbles pseudokinase 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TRIB3
  • Catalog：
    4537
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Tribbles Pseudokinase 3 Recombinant Rabbit mAb
Aliases TRIB3; Tribbles Pseudokinase 3; TRB3; P65-Interacting Inhibitor Of NF-Kappa-B; Tribbles Homolog 3; DJ1103G7.3; C20orf97; SKIP3; TRB-3; NIPK; SINK; Neuronal Cell Death Inducible Putative Kinase; Neuronal Cell Death-Inducible Putative Kinase; P65-Interacting Inhibitor Of NF-KappaB; Chromosome 20 Open Reading Frame 97; Tribbles Homolog 3 (Drosophila); C20ORF97
Background

Gene Name: TRIB3

NCBI Gene Entry: 57761

UniProt Entry: Q96RU7

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11715

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human TRIB3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using tribbles pseudokinase 3 antibody (Cat#4537). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tribbles pseudokinase 3 antibody (Cat#4537, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with Tribbles pseudokinase 3 antibody (Cat#4537, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of tribbles pseudokinase 3 expression in HT-1080 cells using tribbles pseudokinase 3 antibody (Cat#4537, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, tribbles pseudokinase 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TRIB3
  • Catalog：
    4537
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using tribbles pseudokinase 3 antibody (Cat#4537). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tribbles pseudokinase 3 antibody (Cat#4537, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with Tribbles pseudokinase 3 antibody (Cat#4537, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of tribbles pseudokinase 3 expression in HT-1080 cells using tribbles pseudokinase 3 antibody (Cat#4537, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, tribbles pseudokinase 3.
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