Western blotting analysis using ring finger protein 2 antibody (Cat#4769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ring finger protein 2 antibody (Cat#4769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ring finger protein 2 expression in HeLa cells using ring finger protein 2 antibody (Cat#4769, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ring finger protein 2.
RNF2; Ring Finger Protein 2; RING1B; HIPI3; BAP1; DING; BAP-1; RING2; Huntingtin-Interacting Protein 2-Interacting Protein 3; RING-Type E3 Ubiquitin Transferase RING2; E3 Ubiquitin-Protein Ligase RING2; HIP2-Interacting Protein 3; RING Finger Protein BAP-1; RING Finger Protein 1B; Protein DinG; RING Finger Protein; EC 2.3.2.27; EC 6.3.2; LUSYAM; RING1b
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human RING2 / RING1B / RNF2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using ring finger protein 2 antibody (Cat#4769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ring finger protein 2 antibody (Cat#4769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ring finger protein 2 expression in HeLa cells using ring finger protein 2 antibody (Cat#4769, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ring finger protein 2.
Western blotting analysis using ring finger protein 2 antibody (Cat#4769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ring finger protein 2 antibody (Cat#4769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ring finger protein 2 expression in HeLa cells using ring finger protein 2 antibody (Cat#4769, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ring finger protein 2.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
