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RALGAPA1 Recombinant Rabbit mAb

#5246

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Recombinant Antibody
Western blotting analysis using RALGAPA1 antibody (Cat#5246). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALGAPA1 antibody (Cat#5246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RALGAPA1 expression in HeLa cells using RALGAPA1 antibody (Cat#5246, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RALGAPA1.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    RALGAPA1
  • Catalog:
    5246
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name RALGAPA1 Recombinant Rabbit mAb
Aliases RALGAPA1; Ral GTPase Activating Protein Catalytic Subunit Alpha 1; GRIPE; Tuberin-Like Protein 1; RalGAPalpha1; KIAA0884; Tulip1; GARNL1; Ral GTPase-Activating Protein Subunit Alpha-1; GTPase Activating Rap/RanGAP Domain-Like 1; GAP-Related Interacting Protein To E12; DKFZp667F074; P240; Ral GTPase Activating Protein, Alpha Subunit 1 (Catalytic); Ral GTPase Activating Protein Catalytic Alpha Subunit 1; GTPase-Activating Rap/Ran-GAP Domain-Like 1; GTPase Activating RANGAP Domain-Like 1; GAP-Related-Interacting Partner To E12; NEDHRIT; TULIP1
Background

Gene Name: RALGAPA1

NCBI Gene Entry: 253959

UniProt Entry: Q6GYQ0

Application Information

Molecular Weight: Predicted, 230 kDa; observed, 260 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB335

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human RALGAPA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RALGAPA1 antibody (Cat#5246). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALGAPA1 antibody (Cat#5246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of RALGAPA1 expression in HeLa cells using RALGAPA1 antibody (Cat#5246, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RALGAPA1.
Western blotting analysis using RALGAPA1 antibody (Cat#5246). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALGAPA1 antibody (Cat#5246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RALGAPA1 expression in HeLa cells using RALGAPA1 antibody (Cat#5246, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RALGAPA1.
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