Western blotting analysis using phosphoglycerate kinase 1 antibody (Cat#5328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using phosphoglycerate kinase 1 antibody (Cat#5328, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Phosphoglycerate kinase 1 expression in HepG2 cells using Phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red,Phosphoglycerate kinase 1 .
Western blotting analysis using phosphoglycerate kinase 1 antibody (Cat#5328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using phosphoglycerate kinase 1 antibody (Cat#5328, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Phosphoglycerate kinase 1 expression in HepG2 cells using Phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red,Phosphoglycerate kinase 1 .
Western blotting analysis using phosphoglycerate kinase 1 antibody (Cat#5328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using phosphoglycerate kinase 1 antibody (Cat#5328, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Phosphoglycerate kinase 1 expression in HepG2 cells using Phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red,Phosphoglycerate kinase 1 .
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