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Home>Product>Primary Antibody

CFAP298 Mouse mAb

#5395

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Western blotting analysis using CFAP298 antibody (Cat#5395). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CFAP298 antibody (Cat#5395, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CFAP298 antibody (Cat#5395, 1:1,000). Nuclei were stained blue with DAPI; CFAP298 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of CFAP298 expression in HepG2 cells using CFAP298 antibody (Cat#5395, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CFAP298.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CFAP298
  • Catalog：
    5395
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name CFAP298 Mouse mAb
Aliases CFAP298; Cilia And Flagella Associated Protein 298; C21orf48; C21orf59; DNAAF16; CILD26; FBB18; Kur; Cilia- And Flagella-Associated Protein 298; Dynein Axonemal Assembly Factor 16; Protein Kurly Homolog; FLJ20467; Prostate Cancer Upregulated Protein 1; Chromosome 21 Open Reading Frame 48; Chromosome 21 Open Reading Frame 59; Kurly Homolog (Zebrafish); UPF0769 Protein C21orf59; Kurly Homolog
Background

Gene Name: CFAP298

NCBI Gene Entry: 56683

UniProt Entry: P57076

Application Information

Molecular Weight: Predicted, 33 kDa; observed, 33 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1305

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human C21orf59
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CFAP298 antibody (Cat#5395). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CFAP298 antibody (Cat#5395, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with CFAP298 antibody (Cat#5395, 1:1,000). Nuclei were stained blue with DAPI; CFAP298 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Flow cytometric analysis of CFAP298 expression in HepG2 cells using CFAP298 antibody (Cat#5395, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CFAP298.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CFAP298
  • Catalog：
    5395
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CFAP298 antibody (Cat#5395). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CFAP298 antibody (Cat#5395, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CFAP298 antibody (Cat#5395, 1:1,000). Nuclei were stained blue with DAPI; CFAP298 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of CFAP298 expression in HepG2 cells using CFAP298 antibody (Cat#5395, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CFAP298.
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