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Home>Product>Primary Antibody

DECR1 Mouse Oligoclonal Antibody

#5453

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Western blotting analysis using DECR1 antibody (Cat#5453). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DECR1 antibody (Cat#5453, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with DECR1 antibody(Cat#5453, 1:1,000). Nuclei were stained blue with DAPI; DECR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DECR1 expression in HAP-1 cells using DECR1 antibody (Cat#5453, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DECR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DECR1
  • Catalog：
    5453
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name DECR1 Mouse Oligoclonal Antibody
Aliases DECR1; 2,4-Dienoyl-CoA Reductase 1; SDR18C1; DECR; 2,4-Dienoyl-CoA Reductase [(3E)-Enoyl-CoA-Producing], Mitochondrial; Short Chain Dehydrogenase/Reductase Family 18C Member 1; 2,4-Dienoyl-CoA Reductase 1, Mitochondrial; Short Chain Dehydrogenase/Reductase Family 18C, Member 1; 2,4-Dienoyl-CoA Reductase, Mitochondrial; 2,4-Dienoyl-CoA Reductase [NADPH]; 4-Enoyl-CoA Reductase [NADPH]; 4-Enoyl-CoA Reductase; EC 1.3.1.124; NADPH
Background

Gene Name: DECR1

NCBI Gene Entry: 1666

UniProt Entry: Q16698

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 29 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human DECR1
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using DECR1 antibody (Cat#5453). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DECR1 antibody (Cat#5453, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with DECR1 antibody(Cat#5453, 1:1,000). Nuclei were stained blue with DAPI; DECR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DECR1 expression in HAP-1 cells using DECR1 antibody (Cat#5453, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DECR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DECR1
  • Catalog：
    5453
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using DECR1 antibody (Cat#5453). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DECR1 antibody (Cat#5453, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with DECR1 antibody(Cat#5453, 1:1,000). Nuclei were stained blue with DAPI; DECR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DECR1 expression in HAP-1 cells using DECR1 antibody (Cat#5453, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DECR1.
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