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Phospho-Filamin A (S1083) Recombinant Rabbit mAb

#5500

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Recombinant Antibody
Western blotting analysis using phospho-filamin A (S1083) antibody (Cat#5500). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-filamin A (S1083) antibody (Cat#5500, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-Filamin A (S1083) expression in C2C12 cells using Phospho-Filamin A (S1083) antibody (Cat#5500, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Filamin A (S1083).
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FLNA
  • Catalog:
    5500
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name Phospho-Filamin A (S1083) Recombinant Rabbit mAb
Aliases FLNA; Filamin A; ABP-280; FLN1; FLN; Endothelial Actin-Binding Protein; Actin Binding Protein 280; Non-Muscle Filamin; Filamin A, Alpha; Alpha-Filamin; Filamin-A; Filamin-1; FLN-A; OPD1; OPD2; Filamin A, Alpha (Actin Binding Protein 280); Epididymis Secretory Sperm Binding Protein; Actin-Binding Protein 280; Alpha Filamin; EC 2.1.1.43; EC 6.3.4.4; ABPX; CSBS; CVD1; FGS2; NHBP; XLVD; XMVD; FMD; MNS; OPD
Background

Gene Name: FLNA

NCBI Gene Entry: 2316

UniProt Entry: P21333

Application Information

Molecular Weight: Predicted, 281 kDa; observed, 281 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1785

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Phospho-Filamin A (S1083)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-filamin A (S1083) antibody (Cat#5500). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-filamin A (S1083) antibody (Cat#5500, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-Filamin A (S1083) expression in C2C12 cells using Phospho-Filamin A (S1083) antibody (Cat#5500, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Filamin A (S1083).
Western blotting analysis using phospho-filamin A (S1083) antibody (Cat#5500). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-filamin A (S1083) antibody (Cat#5500, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-Filamin A (S1083) expression in C2C12 cells using Phospho-Filamin A (S1083) antibody (Cat#5500, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Filamin A (S1083).
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