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YWHAQ Recombinant Rabbit mAb

#5558

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Recombinant Antibody
Western blotting analysis using YWHAQ antibody (Cat#5558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YWHAQ antibody (Cat#5558, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of YWHAQ expression in HAP-1 cells using YWHAQ antibody (Cat#5558, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, YWHAQ.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    YWHAQ
  • Catalog:
    5558
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name YWHAQ Recombinant Rabbit mAb
Aliases YWHAQ; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Theta; 14-3-3; HS1; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Theta Polypeptide; 14-3-3 Protein T-Cell; 14-3-3 Protein Theta; 14-3-3 Protein Tau; Protein, Theta; 14-3-3 Theta; Protein Tau; Protein HS1; 1C5
Background

Gene Name: YWHAQ

NCBI Gene Entry: 10971

UniProt Entry: P27348

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4250

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human 14-3-3 Theta/Tau
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using YWHAQ antibody (Cat#5558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YWHAQ antibody (Cat#5558, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of YWHAQ expression in HAP-1 cells using YWHAQ antibody (Cat#5558, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, YWHAQ.
Western blotting analysis using YWHAQ antibody (Cat#5558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YWHAQ antibody (Cat#5558, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of YWHAQ expression in HAP-1 cells using YWHAQ antibody (Cat#5558, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, YWHAQ.
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