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Home>Product>Primary Antibody

UBE2I Recombinant Rabbit mAb

#5664

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Recombinant Antibody
Western blotting analysis using UBE2I antibody (Cat#5664). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2I antibody (Cat#5664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2I expression in HepG2 cells using UBE2I antibody (Cat#5664, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UBE2I.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBE2I
  • Catalog：
    5664
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name UBE2I Recombinant Rabbit mAb
Aliases UBE2I; Ubiquitin Conjugating Enzyme E2 I; UBC9; Ubiquitin-Conjugating Enzyme E2I (Homologous To Yeast UBC9); Ubiquitin-Conjugating Enzyme E2I (UBC9 Homolog, Yeast); RING-Type E3 SUMO Transferase UBC9; SUMO-Conjugating Enzyme UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; SUMO-Protein Ligase; P18; Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme; Ubiquitin-Conjugating Enzyme UbcE2A; Ubiquitin-Conjugating Enzyme E2 I; Ubiquitin-Conjugating Enzyme E2I; Ubiquitin Conjugating Enzyme E2I; Ubiquitin Conjugating Enzyme 9; Ubiquitin-Protein Ligase E2I; SUMO-1-Protein Ligase; EC 2.3.2.-; C358B7.1; EC 6.3.2; UBCE9
Background

Gene Name: UBE2I

NCBI Gene Entry: 7329

UniProt Entry: P63279

Application Information

Molecular Weight: Predicted, 18 kDa; observed, 18 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4770

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human UBE2I
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using UBE2I antibody (Cat#5664). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2I antibody (Cat#5664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of UBE2I expression in HepG2 cells using UBE2I antibody (Cat#5664, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UBE2I.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBE2I
  • Catalog：
    5664
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using UBE2I antibody (Cat#5664). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2I antibody (Cat#5664, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2I expression in HepG2 cells using UBE2I antibody (Cat#5664, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UBE2I.
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