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Home>Product>Primary Antibody

GDI1 Mouse mAb

#5697

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Western blotting analysis using GDI1 antibody (Cat#5697). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GDI1 antibody (Cat#5697, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using GDI1 antibody (Cat#5697, 1:5,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of GDI1 expression in HAP-1 cells using GDI1 antibody (Cat#5697, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GDI1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GDI1
  • Catalog：
    5697
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name GDI1 Mouse mAb
Aliases GDI1; GDP Dissociation Inhibitor 1; RABGDIA; OPHN2; Oligophrenin-2; XAP-4; GDIL; Guanosine Diphosphate Dissociation Inhibitor 1; Rab GDP-Dissociation Inhibitor, Alpha; Rab GDP Dissociation Inhibitor Alpha; Protein XAP-4; Rab GDI Alpha; FLJ41411; MRX41; MRX48; GDI-1; Testis Secretory Sperm-Binding Protein Li 208a; Mental Retardation, X-Linked 41; Mental Retardation, X-Linked 48; RABGD1A; XLID41; XAP4; 1A
Background

Gene Name: GDI1

NCBI Gene Entry: 2664

UniProt Entry: P31150

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4950

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human GDI1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:2,500-1:10,000
Note This product is for research use only.
Data
  • Western blotting analysis using GDI1 antibody (Cat#5697). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GDI1 antibody (Cat#5697, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human glioblastoma using GDI1 antibody (Cat#5697, 1:5,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of GDI1 expression in HAP-1 cells using GDI1 antibody (Cat#5697, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GDI1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GDI1
  • Catalog：
    5697
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using GDI1 antibody (Cat#5697). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GDI1 antibody (Cat#5697, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using GDI1 antibody (Cat#5697, 1:5,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of GDI1 expression in HAP-1 cells using GDI1 antibody (Cat#5697, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GDI1.
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