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Home>Product>Primary Antibody

SIL1 Mouse mAb

#5834

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Western blotting analysis using SIL1 antibody (Cat#5834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIL1 antibody (Cat#5834, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with SIL1 antibody(Cat#5834, 1:1,000). Nuclei were stained blue with DAPI; SIL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIL1
  • Catalog：
    5834
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name SIL1 Mouse mAb
Aliases SIL1; SIL1 Nucleotide Exchange Factor ; BAP; ULG5; Nucleotide Exchange Factor SIL1; BiP-Associated Protein; MSS; SIL1 Homolog, Endoplasmic Reticulum Chaperone (S. Cerevisiae); SIL1-Like Protein Endoplasmic Reticulum Chaperone; SIL1 Homolog, Endoplasmic Reticulum Chaperone; Marinesco-Sjogren Syndrome
Background

Gene Name: SIL1

NCBI Gene Entry: 64374

UniProt Entry: Q9H173

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5095

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human SIL1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SIL1 antibody (Cat#5834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIL1 antibody (Cat#5834, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with SIL1 antibody(Cat#5834, 1:1,000). Nuclei were stained blue with DAPI; SIL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIL1
  • Catalog：
    5834
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using SIL1 antibody (Cat#5834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIL1 antibody (Cat#5834, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with SIL1 antibody(Cat#5834, 1:1,000). Nuclei were stained blue with DAPI; SIL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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