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Home>Product>Primary Antibody

PYGM Mouse mAb

#5929

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Western blotting analysis using PYGM antibody (Cat#5929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PYGM antibody (Cat#5929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PYGM expression in HeLa cells using PYGM antibody (Cat#5929, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PYGM.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PYGM
  • Catalog：
    5929
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PYGM Mouse mAb
Aliases PYGM; Glycogen Phosphorylase, Muscle Associated; Glycogen Phosphorylase, Muscle Form; Myophosphorylase; GSD5; EC 2.4.1.1; Glycogen Storage Disease Type V; Phosphorylase, Glycogen; Muscle; Phosphorylase, Glycogen, Muscle; McArdle Syndrome
Background

Gene Name: PYGM

NCBI Gene Entry: 5837

UniProt Entry: P11217

Application Information

Molecular Weight: Predicted, 97 kDa; observed, 95 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5880

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human PYGM
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PYGM antibody (Cat#5929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PYGM antibody (Cat#5929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PYGM expression in HeLa cells using PYGM antibody (Cat#5929, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PYGM.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PYGM
  • Catalog：
    5929
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PYGM antibody (Cat#5929). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PYGM antibody (Cat#5929, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PYGM expression in HeLa cells using PYGM antibody (Cat#5929, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PYGM.
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