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Home>Product>Primary Antibody

Phospho-Hormone Sensitive Lipase (S855) Recombinant Rabbit mAb

#4921

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Recombinant Antibody
Western blotting analysis using phospho-hormone sensitive lipase (Ser855) antibody (Cat#4921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-hormone sensitive lipase (Ser855) antibody (Cat#4921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Phospho-Hormone Sensitive Lipase (Ser855) antibody (Cat#4921, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Hormone Sensitive Lipase (Ser855) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Hormone Sensitive Lipase (Ser855) expression in HAP-1 cells using Phospho-Hormone Sensitive Lipase (Ser855) antibody (Cat#4921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Hormone Sensitive Lipase (Ser855).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LIPE
  • Catalog：
    4921
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Hormone Sensitive Lipase (S855) Recombinant Rabbit mAb
Aliases LIPE; Lipase E, Hormone Sensitive Type; HSL; Monoacylglycerol Lipase LIPE; Lipase, Hormone-Sensitive; Hormone-Sensitive Lipase; Retinyl Ester Hydrolase; EC 3.1.1.79; REH; EC 3.1.1.23; AOMS4; FPLD6; LHS
Background

Gene Name: LIPE

NCBI Gene Entry: 3991

UniProt Entry: Q05469

Application Information

Molecular Weight: Predicted, 117 kDa; observed, 83 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13765

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Hormone Sensitive Lipase (Ser855)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-hormone sensitive lipase (Ser855) antibody (Cat#4921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-hormone sensitive lipase (Ser855) antibody (Cat#4921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with Phospho-Hormone Sensitive Lipase (Ser855) antibody (Cat#4921, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Hormone Sensitive Lipase (Ser855) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-Hormone Sensitive Lipase (Ser855) expression in HAP-1 cells using Phospho-Hormone Sensitive Lipase (Ser855) antibody (Cat#4921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Hormone Sensitive Lipase (Ser855).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LIPE
  • Catalog：
    4921
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-hormone sensitive lipase (Ser855) antibody (Cat#4921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-hormone sensitive lipase (Ser855) antibody (Cat#4921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Phospho-Hormone Sensitive Lipase (Ser855) antibody (Cat#4921, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Hormone Sensitive Lipase (Ser855) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Hormone Sensitive Lipase (Ser855) expression in HAP-1 cells using Phospho-Hormone Sensitive Lipase (Ser855) antibody (Cat#4921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Hormone Sensitive Lipase (Ser855).
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