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Home>Product>Primary Antibody

KD-Validated LRCH4 Mouse mAb

#65902

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shRNA Knockdown Validated
Western blotting analysis using LRCH4 antibody (Cat#65902). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using LRCH4 antibody (Cat#65902). LRCH4 expression in wild-type (WT) and LRCH4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells using LRCH4 antibody (Cat#65902, 1:1,000), Top panel: wild-type (WT); Bottom panal: LRCH4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LRCH4 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of LRCH4 expression in HT-1080 cells using LRCH4 antibody (Cat#65902, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, LRCH4.
Validation of LRCH4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with LRCH4 antibody (Cat#65902, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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  • Gene Symbol：
    LRCH4
  • Catalog：
    65902
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated LRCH4 Mouse mAb
Aliases LRCH4; Leucine Rich Repeats And Calponin Homology Domain Containing 4; LRRN1; LRN; Leucine-Rich Repeat And Calponin Homology Domain-Containing Protein 4; Leucine-Rich Repeats And Calponin Homology (CH) Domain Containing 4; Leucine-Rich Neuronal Protein; LRRN4; Leucine-Rich Repeat Neuronal Protein 4; Leucine Rich Repeat Neuronal 4; PP14183
Background

Gene Name: LRCH4

NCBI Gene Entry: 4034

UniProt Entry: O75427

Application Information

Molecular Weight: Predicted, 73 kDa; observed, 75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5750

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human LRCH4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using LRCH4 antibody (Cat#65902). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using LRCH4 antibody (Cat#65902). LRCH4 expression in wild-type (WT) and LRCH4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells using LRCH4 antibody (Cat#65902, 1:1,000), Top panel: wild-type (WT); Bottom panal: LRCH4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LRCH4 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of LRCH4 expression in HT-1080 cells using LRCH4 antibody (Cat#65902, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, LRCH4.
  • Validation of LRCH4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with LRCH4 antibody (Cat#65902, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LRCH4
  • Catalog：
    65902
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using LRCH4 antibody (Cat#65902). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using LRCH4 antibody (Cat#65902). LRCH4 expression in wild-type (WT) and LRCH4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells using LRCH4 antibody (Cat#65902, 1:1,000), Top panel: wild-type (WT); Bottom panal: LRCH4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LRCH4 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of LRCH4 expression in HT-1080 cells using LRCH4 antibody (Cat#65902, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, LRCH4.
Validation of LRCH4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with LRCH4 antibody (Cat#65902, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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