Western blotting analysis using vesicle associated membrane protein 8 antibody (Cat#61255). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vesicle associated membrane protein 8 antibody (Cat#61255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using vesicle associated membrane protein 8 antibody (Cat#61255). Vesicle associated membrane protein 8 expression in wild-type (WT) and vesicle associated membrane protein 8 (VAMP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with vesicle associated membrane protein 8 antibody (Cat#61255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using vesicle associated membrane protein 8 antibody (Cat#61255, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Vesicle associated membrane protein 8 expression in HepG2 cells using Vesicle associated membrane protein 8 antibody (Cat# 61255, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Vesicle associated membrane protein 8.
Validation of Vesicle associated membrane protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Vesicle associated membrane protein 8 antibody (Cat#61255, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
