Western blotting analysis using collapsin response mediator protein 1 antibody (Cat#61315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with collapsin response mediator protein 1 antibody (Cat#61315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using collapsin response mediator protein 1 antibody (Cat#61315). Collapsin response mediator protein 1 expression in wild-type (WT) and collapsin response mediator protein 1 (CRMP1) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with collapsin response mediator protein 1 antibody (Cat#61315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HSHC cells using collapsin response mediator protein 1 antibody (Cat#61315, 1:1,000), Top panel: wild-type (WT); Bottom panal: Collapsin response mediator protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Collapsin response mediator protein 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of collapsin response mediator protein 1 expression in HAP-1 cells using collapsin response mediator protein 1 antibody (Cat#61315, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Collapsin response mediator protein 1.
Validation of collapsin response mediator protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with collapsin response mediator protein 1 antibody (Cat#61315, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
