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Home>Product>Primary Antibody

Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) Recombinant Rabbit mAb

#1799

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Recombinant Antibody
Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in C2C12 cells using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK3/1
  • Catalog：
    1799
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) Recombinant Rabbit mAb
Aliases MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK 3; MAPK1; Mitogen-Activated Protein Kinase 1; ERK2; Extracellular Signal-Regulated Kinase 2; MAPK2; PRKM1; PRKM2; ERK; Mitogen-Activated Protein Kinase 2; MAP Kinase 1; MAP Kinase 2; EC 2.7.11.24; P42-MAPK; P41mapk; MAPK; ERK-2; ERT1; Protein Tyrosine Kinase; ERK2; MAP Kinase Isoform P42; EC 2.7.11; P42MAPK; P41MAPK; MAPK 1; NS13; P38; P40; P41
Background

Gene Name: MAPK3/1

NCBI Gene Entry: 5595 , 5594

UniProt Entry: P27361 , P28482

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 42,44 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4520

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in C2C12 cells using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK3/1
  • Catalog：
    1799
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in C2C12 cells using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187).
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