Western blotting analysis using phospho-PKC alpha (Ser657) antibody (Cat#4751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with PRKCA antibody (Cat#4751, 1:1,000). Nuclei were stained blue with DAPI; PRKCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PKC alpha (Ser657) expression in HT-1080 cells using Phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PKC alpha (Ser657).
Western blotting analysis using phospho-PKC alpha (Ser657) antibody (Cat#4751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with PRKCA antibody (Cat#4751, 1:1,000). Nuclei were stained blue with DAPI; PRKCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PKC alpha (Ser657) expression in HT-1080 cells using Phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PKC alpha (Ser657).
Western blotting analysis using phospho-PKC alpha (Ser657) antibody (Cat#4751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with PRKCA antibody (Cat#4751, 1:1,000). Nuclei were stained blue with DAPI; PRKCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PKC alpha (Ser657) expression in HT-1080 cells using Phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PKC alpha (Ser657).
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