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Home>Product>Primary Antibody

Paired box 6 Recombinant Rabbit mAb

#4247

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Recombinant Antibody
Western blotting analysis using paired box 6 antibody (Cat#4247). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with paired box 6 antibody (Cat#4247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using paired box 6 antibody (Cat#4247, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Paired box 6 expression in HeLa cells using Paired box 6 antibody (Cat#4247, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Paired box 6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAX6
  • Catalog：
    4247
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name Paired box 6 Recombinant Rabbit mAb
Aliases PAX6; Paired Box 6; D11S812E; WAGR; AN2; AN; Paired Box Protein Pax-6; Aniridia Type II Protein; Oculorhombin; Aniridia 1; Aniridia 2; AN1; Paired Box Gene 6 (Aniridia, Keratitis); Paired Box 6 PAX6A-ASdel6-AS7 Isoform; Paired Box 6 PAX6A-ASdel6 Isoform; Paired Box 6 PAX6B-ASdel6 Isoform; Paired Box 6 PAX6A-Del6 Isoform; Paired Box 6 PAX6-12a Isoform; Paired Box 6 PAX6D Isoform; Paired Box Homeotic Gene-6; Alternative Protein PAX6; Aniridia, Keratitis; ASGD5; FVH1; MGDA
Background

Gene Name: PAX6

NCBI Gene Entry: 5080

UniProt Entry: P26367

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10265

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human PAX6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using paired box 6 antibody (Cat#4247). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with paired box 6 antibody (Cat#4247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using paired box 6 antibody (Cat#4247, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Paired box 6 expression in HeLa cells using Paired box 6 antibody (Cat#4247, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Paired box 6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PAX6
  • Catalog：
    4247
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using paired box 6 antibody (Cat#4247). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with paired box 6 antibody (Cat#4247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using paired box 6 antibody (Cat#4247, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Paired box 6 expression in HeLa cells using Paired box 6 antibody (Cat#4247, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Paired box 6.
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