Western blotting analysis using Casein kinase 2 beta antibody (Cat#61434). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Casein kinase 2 beta antibody (Cat#61434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Casein kinase 2 beta antibody (Cat#61434). Casein kinase 2 beta expression in wild type (WT) and Casein kinase 2 beta shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Casein kinase 2 beta antibody (Cat#61434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Casein kinase 2 beta antibody (Cat#61434, 1:1,000). Nuclei were stained blue with DAPI; Casein kinase 2 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Casein kinase 2 beta antibody (Cat#61434, 1:1,000), Top panel: wild-type (WT); Bottom panal: Casein kinase 2 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 2 beta was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
