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Home>Product>Primary Antibody

KD-Validated CNOT4 Mouse mAb

#61542

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Knockdown Validated
Western blotting analysis using CNOT4 antibody (Cat#61542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNOT4 antibody (Cat#61542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CNOT4 antibody (Cat#61542). CNOT4 expression in wild-type (WT) and CNOT4 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CNOT4 antibody (Cat#61542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CNOT4 antibody (Cat#61542, 1:1,000). Nuclei were stained blue with DAPI; CNOT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    CNOT4
  • Catalog：
    61542
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated CNOT4 Mouse mAb
Aliases CNOT4;CCR4-NOT Transcription Complex Subunit 4; CLONE243; NOT4H; NOT4; Potential Transcriptional Repressor NOT4Hp; RING-Type E3 Ubiquitin Transferase CNOT4; E3 Ubiquitin-Protein Ligase CNOT4; CCR4-Associated Factor 4; NOT4 (Negative Regulator Of Transcription 4, Yeast) Homolog; CCR4-NOT Transcription Complex, Subunit 4; EC 2.3.2.27
Background

Gene Name: CNOT4

NCBI Gene Entry: 4850

UniProt Entry: O95628

Application Information

Molecular Weight: Predicted, 64 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB2750

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human CNOT4
Isotype Mouse IgG2a kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CNOT4 antibody (Cat#61542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNOT4 antibody (Cat#61542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using CNOT4 antibody (Cat#61542). CNOT4 expression in wild-type (WT) and CNOT4 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CNOT4 antibody (Cat#61542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with CNOT4 antibody (Cat#61542, 1:1,000). Nuclei were stained blue with DAPI; CNOT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CNOT4
  • Catalog：
    61542
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CNOT4 antibody (Cat#61542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNOT4 antibody (Cat#61542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CNOT4 antibody (Cat#61542). CNOT4 expression in wild-type (WT) and CNOT4 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CNOT4 antibody (Cat#61542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CNOT4 antibody (Cat#61542, 1:1,000). Nuclei were stained blue with DAPI; CNOT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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