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Home>Product>Primary Antibody

KD-Validated Sonic Hedgehog Signaling Molecule Recombinant Rabbit mAb

#62804

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using sonic hedgehog signaling molecule antibody (Cat#62804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sonic hedgehog signaling molecule antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using sonic hedgehog signaling molecule antibody (Cat#62804). Sonic hedgehog signaling molecule expression in wild-type (WT) and sonic hedgehog signaling molecule (SHH) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with sonic hedgehog signaling molecule antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using sonic hedgehog signaling molecule antibody (Cat#62804, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with sonic hedgehog signaling molecule antibody (Cat#62804, 1:1,000). Nuclei were stained blue with DAPI; Sonic hedgehog signaling molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of sonic hedgehog signaling molecule expression in HepG2 cells using sonic hedgehog signaling molecule antibody (Cat#62804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sonic hedgehog signaling molecule.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SHH
  • Catalog：
    62804
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Sonic Hedgehog Signaling Molecule Recombinant Rabbit mAb
Aliases SHH; Sonic Hedgehog Signaling Molecule; MCOPCB5; SMMCI; TPTPS; HHG1; TPT; Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains; Sonic Hedgehog Protein; ShhNC; HLP3; HPE3; Sonic Hedgehog (Drosophila) Homolog; Sonic Hedgehog Homolog; EC 3.1.-.-; HHG-1
Background

Gene Name: SHH

NCBI Gene Entry: 6469

UniProt Entry: Q15465

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 51 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1925

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Sonic Hedgehog
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using sonic hedgehog signaling molecule antibody (Cat#62804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sonic hedgehog signaling molecule antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using sonic hedgehog signaling molecule antibody (Cat#62804). Sonic hedgehog signaling molecule expression in wild-type (WT) and sonic hedgehog signaling molecule (SHH) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with sonic hedgehog signaling molecule antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using sonic hedgehog signaling molecule antibody (Cat#62804, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with sonic hedgehog signaling molecule antibody (Cat#62804, 1:1,000). Nuclei were stained blue with DAPI; Sonic hedgehog signaling molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of sonic hedgehog signaling molecule expression in HepG2 cells using sonic hedgehog signaling molecule antibody (Cat#62804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sonic hedgehog signaling molecule.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SHH
  • Catalog：
    62804
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using sonic hedgehog signaling molecule antibody (Cat#62804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with sonic hedgehog signaling molecule antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using sonic hedgehog signaling molecule antibody (Cat#62804). Sonic hedgehog signaling molecule expression in wild-type (WT) and sonic hedgehog signaling molecule (SHH) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with sonic hedgehog signaling molecule antibody (Cat#62804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using sonic hedgehog signaling molecule antibody (Cat#62804, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with sonic hedgehog signaling molecule antibody (Cat#62804, 1:1,000). Nuclei were stained blue with DAPI; Sonic hedgehog signaling molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of sonic hedgehog signaling molecule expression in HepG2 cells using sonic hedgehog signaling molecule antibody (Cat#62804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Sonic hedgehog signaling molecule.
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