Western blotting analysis using syntaxin 4 antibody (Cat#61691). Syntaxin 4 expression in wild-type (WT) and syntaxin 4 (STX4) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with syntaxin 4 antibody (Cat#61691, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Syntaxin 4 expression in HepG2 cells using Syntaxin 4 antibody (Cat#61691, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Syntaxin 4.
Western blotting analysis using syntaxin 4 antibody (Cat#61691). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with syntaxin 4 antibody (Cat#61691, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
