KD-Validated PPP1R1B Recombinant Rabbit mAb - Product

KD-Validated PPP1R1B Recombinant Rabbit mAb

#61748

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using PPP1R1B antibody (Cat#61748). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1R1B antibody (Cat#61748, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using PPP1R1B antibody (Cat#61748). PPP1R1B expression in wild-type (WT) and PPP1R1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PPP1R1B antibody (Cat#61748, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPP1R1B
Catalog：

61748
Application：

WB
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated PPP1R1B Recombinant Rabbit mAb
Aliases	PPP1R1B; Protein Phosphatase 1 Regulatory Inhibitor Subunit 1B; DARPP-32; Protein Phosphatase 1 Regulatory Subunit 1B; FLJ20940; DARPP32; Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B; Dopamine And CAMP-Regulated Neuronal Phosphoprotein 32; Dopamine- And CAMP-Regulated Neuronal Phosphoprotein; Dopamine And CAMP Regulated Phosphoprotein
Background	Gene Name: PPP1R1B NCBI Gene Entry: 84152 UniProt Entry: Q9UD71
Application Information	Molecular Weight: Predicted, 23 kDa; observed, 32 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB3540 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB)
Immunogen	A synthesized peptide derived from human DARPP32
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000
Note	This product is for research use only.

Data

Western blotting analysis using PPP1R1B antibody (Cat#61748). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1R1B antibody (Cat#61748, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PPP1R1B antibody (Cat#61748). PPP1R1B expression in wild-type (WT) and PPP1R1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PPP1R1B antibody (Cat#61748, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PPP1R1B
Catalog：

61748
Application：

WB
Species Reactivity：

H, M, R

Datasheet

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