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Home>Product>Primary Antibody

Zinc Finger FYVE-Type Containing 9 Recombinant Rabbit mAb

#2689

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Recombinant Antibody
Western blotting analysis using ZFYVE9 antibody (Cat#2689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZFYVE9 antibody (Cat#2689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with ZFYVE9 antibody (Cat#2689, 1:1,000). Nuclei were stained blue with DAPI; ZFYVE9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ZFYVE9 expression in C2C12 cells using ZFYVE9 antibody (Cat#2689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZFYVE9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ZFYVE9
  • Catalog：
    2689
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Zinc Finger FYVE-Type Containing 9 Recombinant Rabbit mAb
Aliases ZFYVE9; Zinc Finger FYVE-Type Containing 9; SMADIP; SARA; PPP1R173; MADHIP; Protein Phosphatase 1, Regulatory Subunit 173; Zinc Finger FYVE Domain-Containing Protein 9; Zinc Finger, FYVE Domain Containing 9; Smad Anchor For Receptor Activation; Novel Serine Protease; NSP; MAD, Mothers Against Decapentaplegic Homolog (Drosophila) Interacting Protein, Receptor Activation Anchor; MAD, Mothers Against Decapentaplegic Homolog Interacting Protein, Receptor Activation Anchor; Mothers Against Decapentaplegic Homolog-Interacting Protein; Receptor Activation Anchor; MADH-Interacting Protein; Madh-Interacting Protein; HSARA
Background

Gene Name: ZFYVE9

NCBI Gene Entry: 9372

UniProt Entry: O95405

Application Information

Molecular Weight: Predicted, 156 kDa; observed, 180 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1885

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ZFYVE9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ZFYVE9 antibody (Cat#2689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZFYVE9 antibody (Cat#2689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with ZFYVE9 antibody (Cat#2689, 1:1,000). Nuclei were stained blue with DAPI; ZFYVE9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ZFYVE9 expression in C2C12 cells using ZFYVE9 antibody (Cat#2689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZFYVE9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ZFYVE9
  • Catalog：
    2689
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ZFYVE9 antibody (Cat#2689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZFYVE9 antibody (Cat#2689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with ZFYVE9 antibody (Cat#2689, 1:1,000). Nuclei were stained blue with DAPI; ZFYVE9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ZFYVE9 expression in C2C12 cells using ZFYVE9 antibody (Cat#2689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZFYVE9.
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