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Home>Product>Primary Antibody

KD-Validated CYP1A2 Mouse mAb

#61822

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Knockdown Validated
Western blotting analysis using CYP1A2 antibody (Cat#61822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP1A2 antibody (Cat#61822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CYP1A2 antibody (Cat#61822). CYP1A2 expression in wild-type (WT) and CYP1A2 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CYP1A2 antibody (Cat#61822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CYP1A2 expression in HAP-1 cells using CYP1A2 antibody (Cat#61822, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP1A2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP1A2
  • Catalog：
    61822
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated CYP1A2 Mouse mAb
Aliases CYP1A2; Cytochrome P450 Family 1 Subfamily A Member 2; P3-450; CP12; Cytochrome P450, Subfamily I (Aromatic Compound-Inducible), Polypeptide 2; Cytochrome P450, Family 1, Subfamily A, Polypeptide 2; Hydroperoxy Icosatetraenoate Dehydratase; Cholesterol 25-Hydroxylase; Cytochrome P450 1A2; Cytochrome P(3)450; Cytochrome P450-P3; Cytochrome P450 4; EC 1.14.14.1; CYPIA2; Flavoprotein-Linked Monooxygenase; Aryl Hydrocarbon Hydroxylase; Microsomal Monooxygenase; Xenobiotic Monooxygenase; Dioxin-Inducible P3-450; EC 4.2.1.152; P450 Form 4; P450(PA)
Background

Gene Name: CYP1A2

NCBI Gene Entry: 1544

UniProt Entry: P05177

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 59 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3895

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Cytochrome P450 1A2
Isotype Mouse IgG3
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYP1A2 antibody (Cat#61822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP1A2 antibody (Cat#61822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using CYP1A2 antibody (Cat#61822). CYP1A2 expression in wild-type (WT) and CYP1A2 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CYP1A2 antibody (Cat#61822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of CYP1A2 expression in HAP-1 cells using CYP1A2 antibody (Cat#61822, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP1A2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP1A2
  • Catalog：
    61822
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CYP1A2 antibody (Cat#61822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP1A2 antibody (Cat#61822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CYP1A2 antibody (Cat#61822). CYP1A2 expression in wild-type (WT) and CYP1A2 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CYP1A2 antibody (Cat#61822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CYP1A2 expression in HAP-1 cells using CYP1A2 antibody (Cat#61822, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP1A2.
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