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Home>Product>Primary Antibody

KD-Validated LEF1 Mouse mAb

#61834

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Knockdown Validated
Western blotting analysis using LEF1 antibody (Cat#61834). LEF1 expression in wild-type (WT) and LEF1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LEF1 antibody (Cat#61834, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with LEF1 antibody (Cat#61834, 1:1,000). Nuclei were stained blue with DAPI; LEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of LEF1 expression in HAP-1 cells using LEF1 antibody (Cat#61834, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, LEF1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LEF1
  • Catalog：
    61834
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated LEF1 Mouse mAb
Aliases LEF1; Lymphoid Enhancer Binding Factor 1; Lymphoid Enhancer-Binding Factor 1; TCF1ALPHA; TCF7L3; TCF10; T Cell-Specific Transcription Factor 1-Alpha; TCF1-Alpha; LEF-1
Background

Gene Name: LEF1

NCBI Gene Entry: 51176

UniProt Entry: Q9UJU2

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3940

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human LEF1
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using LEF1 antibody (Cat#61834). LEF1 expression in wild-type (WT) and LEF1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LEF1 antibody (Cat#61834, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with LEF1 antibody (Cat#61834, 1:1,000). Nuclei were stained blue with DAPI; LEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of LEF1 expression in HAP-1 cells using LEF1 antibody (Cat#61834, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, LEF1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LEF1
  • Catalog：
    61834
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using LEF1 antibody (Cat#61834). LEF1 expression in wild-type (WT) and LEF1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LEF1 antibody (Cat#61834, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with LEF1 antibody (Cat#61834, 1:1,000). Nuclei were stained blue with DAPI; LEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of LEF1 expression in HAP-1 cells using LEF1 antibody (Cat#61834, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, LEF1.
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