Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated Phospho-CBL (Y774) Recombinant Rabbit mAb

#61989

Click to copy product information

Return to List

Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using phospho-CBL (Y774) antibody (Cat#61989). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CBL (Y774) antibody (Cat#61989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-CBL (Y774) antibody (Cat#61989). Phospho-CBL (Y774) expression in wild-type (WT) and CBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CBL (Y774) antibody (Cat#61989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-CBL (Y774) expression in HT-1080 cells using Phospho-CBL (Y774) antibody (Cat#61989, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-CBL (Y774).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CBL
  • Catalog：
    61989
  • Application：
    WB, FCM
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated Phospho-CBL (Y774) Recombinant Rabbit mAb
Aliases CBL; Cbl Proto-Oncogene; RNF55; C-Cbl; CBL2; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence; Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase; Casitas B-Lineage Lymphoma Proto-Oncogene; RING-Type E3 Ubiquitin Transferase CBL; E3 Ubiquitin-Protein Ligase CBL; Signal Transduction Protein CBL; RING Finger Protein 55; Proto-Oncogene C-Cbl; Oncogene CBL2; Fragile Site, Folic Acid Type, Rare, Fra(11)(Q23.3); EC 2.3.2.27; EC 6.3.2; FRA11B; NSLL
Background

Gene Name: CBL

NCBI Gene Entry: 867

UniProt Entry: P22681

Application Information

Molecular Weight: Predicted, 100 kDa; observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7555

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Phospho-CBL (Y774)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-CBL (Y774) antibody (Cat#61989). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CBL (Y774) antibody (Cat#61989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using phospho-CBL (Y774) antibody (Cat#61989). Phospho-CBL (Y774) expression in wild-type (WT) and CBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CBL (Y774) antibody (Cat#61989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-CBL (Y774) expression in HT-1080 cells using Phospho-CBL (Y774) antibody (Cat#61989, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-CBL (Y774).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CBL
  • Catalog：
    61989
  • Application：
    WB, FCM
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using phospho-CBL (Y774) antibody (Cat#61989). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CBL (Y774) antibody (Cat#61989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-CBL (Y774) antibody (Cat#61989). Phospho-CBL (Y774) expression in wild-type (WT) and CBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CBL (Y774) antibody (Cat#61989, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-CBL (Y774) expression in HT-1080 cells using Phospho-CBL (Y774) antibody (Cat#61989, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-CBL (Y774).
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.