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Home>Product>Primary Antibody

KD-Validated IFIH1 Mouse mAb

#62077

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shRNA Knockdown Validated
Western blotting analysis using IFIH1 antibody (Cat#62077). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIH1 antibody (Cat#62077, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using IFIH1 antibody (Cat#62077). IFIH1 expression in wild-type (WT) and IFIH1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IFIH1 antibody (Cat#62077, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IFIH1
  • Catalog：
    62077
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated IFIH1 Mouse mAb
Aliases IFIH1; Interferon Induced With Helicase C Domain 1; MDA-5; MDA5; Helicard; IDDM19; Hlcd; Interferon-Induced Helicase C Domain-Containing Protein 1; Clinically Amyopathic Dermatomyositis Autoantigen 140 KDa; Melanoma Differentiation-Associated Protein 5; Melanoma Differentiation-Associated Gene 5; RNA Helicase-DEAD Box Protein 116; Murabutide Down-Regulated Protein; Helicase With 2 CARD Domain; RIG-I-Like Receptor 2; CADM-140 Autoantigen; RLR-2; Interferon-Induced With Helicase C Domain Protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide; EC 3.6.4.13; SGMRT1; IMD95; RH116; AGS7
Background

Gene Name: IFIH1

NCBI Gene Entry: 64135

UniProt Entry: Q9BYX4

Application Information

Molecular Weight: Predicted, 117 kDa; observed, 120 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9815

Species Reactivity: Human

Applications Tested: Western Blotting (WB)
Immunogen Recombinant protein of human IFIH1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using IFIH1 antibody (Cat#62077). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIH1 antibody (Cat#62077, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using IFIH1 antibody (Cat#62077). IFIH1 expression in wild-type (WT) and IFIH1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IFIH1 antibody (Cat#62077, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IFIH1
  • Catalog：
    62077
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using IFIH1 antibody (Cat#62077). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIH1 antibody (Cat#62077, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using IFIH1 antibody (Cat#62077). IFIH1 expression in wild-type (WT) and IFIH1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IFIH1 antibody (Cat#62077, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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